introduction

In the 1980s, environmental scientists gradually discovered a series of problems that hindered sustainable development, such as ecological and environmental pollution caused by human over reliance on pesticides and fertilizers in agricultural production. The meeting emphasized that establishing a sound green, low-carbon and circular development economic system, promoting comprehensive green transformation of economic and social development, is the fundamental strategy to solve China's resource, environmental and ecological problems. This conference marks that China's green development has become an important basic national policy. As early as 2005, General Secretary Xi Jinping, who was then the Secretary of the Zhejiang Provincial Party Committee, summarized the concept of green development that green mountains and clear waters are as valuable as mountains of gold and silver. Practice has also fully proved that a good ecological environment is a prerequisite for human survival and the foundation of economic development.

As an old industrial base, Liaoning has enormous room for improvement in natural ecological environment protection, restoration of abandoned mining areas, restoration of soil biological vitality in farmland, and green planting and breeding. Microbial experts from enterprises and cooperative research institutions such as China Institute of Applied Ecology and Liaoning Academy of Agricultural Sciences in the fields of environmental remediation and green food production have profound technical reserves and will play an irreplaceable and enormous role in the new era of green development concept.

1、 The role of microbial agents in increasing yield and improving the quality of major grains, vegetables, and fruits

The live bacteria and their metabolites in scientifically formulated microbial agents play a comprehensive role in the agricultural ecosystem. For example, Bacillus subtilis and Bacillus licheniformis actinomycetes have the ability to hydrolyze phosphorus, potassium, and nitrogen, which can increase the release of phosphorus and potassium in the soil and fix nitrogen in the air, reduce the use of fertilizers, and play a significant role in the nutrient supply of crops in the middle and later stages. Therefore, they have a significant yield increasing effect on crops. At the same time, metabolites in microbial fertilizers can improve plant photosynthetic efficiency, accelerate plant growth and development processes, promote root development, and most importantly, increase rhizosphere secretions, providing sufficient nutrition for rhizosphere microorganisms and greatly promoting the quantity and activity of rhizosphere inoculated microorganisms. These are all guarantees for ensuring early maturity, increased yield, and improved quality of crops.

1. Application and Effect of Microbial Agents in Rice Planting

Microbial agents have shown outstanding effects on increasing yield and improving the quality of rice. Throughout the entire growing season of rice, the effectiveness of microbial agents can be clearly demonstrated. After the first use before transplanting, the rice seedlings showed extremely strong vitality, and there was no obvious slow seedling stage compared to the control. They directly entered the growth stage. In case of early spring low temperature weather, the tillering process in the early stage was not affected, and the root plate growth was more than 50% higher than the control. The nitrogen and phosphorus utilization efficiency in the seedling stage increased by 60% and 40%, the number of mature panicles was more than 10% higher than the control, the rice yield increased by 10%, and the maturity was about 10 days earlier. Generally, the yield of the plot increased by 30-50%. In years with early frost, the yield increase was greater than that of the control.

2. Application and Effect of Microbial Agents in Corn Planting

Wrapping microbial agents around seeds during sowing can significantly increase seed vitality and resistance to spring low temperatures, increase seed germination rate by 20%, reach total leaf count one week earlier than the control in the early growth stage, promote robust plant development, plump corn stalks, and increase yield by 15-30%.

3. Application and effect of microbial agents in soybean and peanut planting

Microbial agents and their metabolites, when used as seed coating agents or in conjunction with sowing applications, have similar effects on soybeans and peanuts, significantly increasing germination rate, yield, and early maturation. On soybeans, it shows an increase of about twice the number of root nodules. Due to early maturation and faster grain filling, the hardening of soybean grains is accelerated, and the rate of pest damage is significantly reduced, resulting in a 100% increase in comprehensive benefits; Peanut yields increase by 60-70%, achieving the yield of plastic film covering without increasing costs, and significantly improving economic benefits.

4. Application and Effect of Microbial Agents in Fruit Planting

Microbial agents have unique effects on activating fruit tree soil, promoting root development, and improving fruit quality. Grapes mature 10-15 days earlier, with fruit size increasing by 15-30%, eliminating small grains, increasing sugar content by 23%, and exhibiting excellent flavor. In addition, similar effects have been achieved in major fruit types such as blueberries, apples, and pears.

5. Application and Effect of Microbial Agents in Vegetable Planting

Due to the large amount of fertilizer applied in vegetable fields, the soil is generally characterized by excessive fertilizer (salinization) and frequent occurrence of diseases. So vegetable production needs to address both underground and above ground issues simultaneously. Underground soil salinization needs to be addressed by applying microbial agents to improve soil fertility, which can better release accumulated nutrients such as phosphorus and potassium. Microorganisms can also passivate heavy metals and reduce pathogen pollution. For above ground plants, microbial agents not only increase yield but also significantly improve flavor compounds and nutrition. They can also enhance disease resistance and reduce the use of fertilizers and pesticides. The company's products have shown good performance on tomatoes, cucumbers, peppers, cowpeas, radishes, and various leafy vegetables, with a yield increase of over 20-90% and significant improvement in taste.

2、 The role of microbial agents in environmental and agricultural pollution control

For most of the soil erosion, livestock and household waste, heavy metal pollution, etc., specific microorganisms, mycorrhizal fungi, and metabolites of specific microorganisms can be used for remediation and treatment to restore vitality to damaged or severely polluted land. The application of microbial agents in environmental pollution ecological restoration and farmland management is very extensive. The following will elaborate on several typical important areas.

1. Greening restoration in mining areas
The greening project in mining areas and landslide areas is a complex ecological engineering technology. Simple planting of grass and trees is difficult to achieve ideal results. It is necessary to add sufficient amounts of microorganisms and their metabolites to the new soil layer to activate the soil and stimulate plant growth, so that the fixed vegetation can survive better. Green barren mining areas or landslides in the shortest possible time.

2. Water pollution control in river basins and urban ditches

River pollution comes from the loss of fertilizers from planting, emissions from animal husbandry, urban and rural domestic sewage, industrial sewage, etc. Except for the Yalu River, the majority of rivers in Liaoning have only Class 5 water quality standards in the middle section, making it difficult to see the beautiful scenery of green mountains and clear waters. With the increase of urbanization, the discharge of domestic sewage from urban ditches has become seriously polluted. Despite the existence of urban sewage treatment systems, the discharge of water from urban ditches far exceeds the prescribed discharge standards, and the odor is pervasive. Mosquitoes and flies fly around, making it difficult to improve the urban living environment with existing measures. So the focus of river pollution control is on the treatment of pollution sources, which will be discussed in later chapters. For river basins and urban and rural ditches, the release of highly active microbial metabolites can promote the growth of aquatic plants and algae, accelerate the metabolism of organic pollutants such as nitrogen and phosphorus (BOD) in water, and achieve the effect of clean water quality.

3. Management of fertilizer loss in agriculture

Using microbial agents in farmland within a 10 kilometer radius of the Great River Basin can reduce nitrogen and phosphorus emissions by more than 50% by improving the utilization efficiency of crops for nitrogen and phosphorus fertilizers, greatly reducing the pollution of river water and groundwater caused by agricultural production. Plant grass and trees near the river, establish artificial wetlands, and use microbial agents to maintain wetland plants, minimizing the pollution of the river caused by fertilizer loss in the planting industry.

4. Fecal pollution control and resource utilization in animal husbandry

With the growth of meat consumption among Chinese residents, aquaculture has become the biggest source of agricultural pollution. Breeding pollution accounts for over 90% of the total agricultural non-point source emissions (i.e. pollution without fixed discharge outlets), with an annual discharge of over 4 billion tons of manure. Even if all of it is used to prepare organic fertilizers, it exceeds the carrying capacity of existing farmland. If these excreta are not treated harmlessly, they will seriously pollute the surrounding environment and water sources. So the manure from animal husbandry also means the greatest waste of resources before the resource utilization is completed. The current sewage treatment technology has problems such as low conversion efficiency, high energy consumption, and poor economic benefits, which restrict the market-oriented development of pollutant treatment. In the treatment of pig, cow, sheep, and chicken manure, pig manure is the most suitable method for biogas treatment. The end of pipe biogas slurry treatment can be combined with the "Simple Straw Temperature Control Fermentation Technology" to achieve zero pollution emissions throughout the entire treatment process (including the breeding process). Cattle, sheep, and chicken manure are suitable for direct combination with straw treatment, and can be transformed into resources through the "Simple Straw Temperature Controlled Fermentation Technology". The so-called "Simple Straw Temperature Controlled Fermentation Technology" fermentation bed is a medium low temperature controlled fermentation technology that can transform organic matter in feces and straw through medium high temperature microbial fermentation, while removing harmful substances and bacteria. Its output is the culture material for grass rot edible fungi (such as Agaricus bisporus, Agaricus bisporus, Agaricus bisporus, and Fusarium oxysporum). The cultivation of grass rot fungi in Liaoning lags far behind that of wood rot fungi, and there is huge room for development. Compared with the aquaculture industry, it can create a grass rot mushroom edible mushroom industry with comparable benefits. The products can not only supply the domestic market but also the international market. Due to the lack of large-scale and specialized fermentation enterprises for comprehensive treatment of straw manure in China, there is no supply of high-quality grass rot fungus culture materials in the market. It is also difficult for small-scale cultivation material preparation units to produce high-quality grass rot fungus cultivation materials. The yield of shiitake mushrooms per unit area in China is only one fourth of that in the Netherlands, so combining the "Simple Straw Temperature Controlled Fermentation Technology" with the treatment of livestock manure and straw has broad market application prospects. In terms of biogas technology, Deshen Enterprise's low-temperature biogas bacteria and secondary fermentation technology can greatly improve the economic benefits of pig manure treatment, achieving zero emissions and 100% resource utilization of pig manure treatment. The nationwide utilization of pig manure biogas can produce approximately 400 billion cubic meters of natural gas annually, which is equivalent to the existing natural gas supply. The resource utilization of pollutants in the aquaculture industry is a great undertaking that benefits the country and the people for generations to come, and is the most important manifestation of implementing the Party Central Committee's "Guiding Opinions on Accelerating the Establishment and Improvement of a Green, Low Carbon, and Circular Development Economic System".

5. Control of heavy metal pollution in farmland

Cadmium pollution in rice is a difficult problem in current grain production, especially in southern rice regions. Due to widespread cadmium pollution, China has been forced to focus its main grain production areas on northern rice regions. The microbial agents produced by Deshen contain unique microbial metabolites that regulate the activity of rice iron sulfur protease, preventing cadmium from transferring from the soil to the grains (but the roots are enriched with a large amount of cadmium, which has not been published in the article). This can produce rice that meets the standard in soils with existing cadmium pollution levels. After rice harvest, removing the root system enriched in cadmium can achieve the effect of reducing soil cadmium content year by year and achieve the cure of cadmium pollution. Live microorganisms in microbial preparations can also passivate various harmful metals in soil. At the same time, it can also promote a significant increase in rice production, improve farmers' income, and better achieve China's staple food security.

3、 The role of microbial agents in biological feed additives for livestock, poultry and aquatic products
Microorganisms also play a crucial role in the growth and development of animals. It plays an indispensable role in digestion, improving immunity, and preventing diseases and

pests, and is an important alternative product for reducing antibiotics and improving meat quality in the breeding industry.
The breeding technology of microbial feed additives for livestock and poultry is to use modern biotechnology to produce live microbial preparations containing a large amount of beneficial bacteria (Bacillus subtilis, yeast, lactic acid bacteria, photosynthetic bacteria, etc.) and their metabolites and growth promoting factors (various vitamins, organic acids, amino acids, proteases, amylase, lipase, amylase, etc.) as feed additives for consumption by livestock and poultry. After being consumed by livestock and poultry, microorganisms can develop and reproduce in the digestive tract, especially in the intestine, to maintain the balance of gut microbiota. By increasing or greatly reducing enzyme system activity, the body's metabolic processes can be improved, which can promote the growth and development of livestock and poultry, improve feed utilization efficiency, reduce the mortality rate of young livestock and poultry, prevent digestive diseases, and enhance the body's immunity.

The application technology of microorganisms in the aquaculture industry has become increasingly mature. Except for individual links that require antibiotics (which do not affect overall quality), microbial and other biological agents (including vaccines) can solve the prevention and control of diseases and pests throughout the entire aquaculture process. Liaoning Deshen Microbiology Co., Ltd. has collaborated with the Shenyang Institute of Applied Ecology of the Chinese Academy of Sciences to organize professionals to conduct extensive research and development work. Our company's edible microbial feed additive has been widely applied and achieved good results. The breeding technology of microbial feed additives for livestock and poultry is at the forefront of scientific and technological fields, and major domestic newspapers and magazines have also promoted it from various aspects and perspectives, currently gaining recognition from a large number of farmers. At present, this technology is in the stage of further research, experimentation, and promotion and application.

Microbial agents, a new type of biological preparation, are influencing the increasingly developing livestock industry with their unique functions. With the further deepening of microecological research and the increasing awareness of green, safe, and environmental protection among people, the application prospects of microorganisms will be even broader.

1. The role of microbial agents in feed for meat chickens and laying hens
The use of microbial agents and animal mulberry yellow can increase the breeding rate by 5.04-5.66%; The egg production rate of poultry increased by 2.15-2.68%, cholesterol in eggs decreased by 70%, and lecithin increased threefold, significantly improving the nutritional value of eggs. By administering vaccines, providing gut microbiota agents in the early stages, and providing animal mulberry yellow in the middle and later stages, the immune system of poultry can be improved, and the use of antibiotics and antiviral drugs can be reduced by 95%.

2. The role of microbial agents in biological feed for pigs

Application has shown that microbial agents have a significant growth promoting effect. The experimental group using microbial agents did not experience diarrhea symptoms, while the control group had a diarrhea rate of 19%. This proves that microbial agents can effectively regulate microbial balance when entering animal intestines and exhibit significant anti diarrheal effects. Feeding pigs with microbial agents can effectively improve the meat to feed ratio, increase feed utilization rate by 3.5-6.6%, increase daily weight gain by more than 5%, reduce costs, greatly improve pork quality, and significantly increase economic benefits. Adding microbial agents to pig farming, due to the lack of antibiotics, no pollution, healthy pigs, and good pork quality, can increase the price of a pig by about 500-1000 yuan compared to ordinary pigs. Microorganisms purify toxic substances in the intestines of pigs, resulting in odorless feces, improved feeding environment, and no maggots or flies.

3. The role of microbial agents in biological feed for cattle

Through experimental comparison, it was found that consuming microbial agents improved the palatability and feed utilization rate of dairy cows, thereby promoting digestion and absorption. The average yield of dairy cows in the experimental group increased by 0.885 kilograms, and the daily net income per cow increased by more than 2.65 yuan. The daily weight gain of beef cattle significantly increased, and the diarrhea rate decreased by 26.65-35.68%.

4. The role of microbial agents in aquatic feed

In the process of development, China's aquaculture industry has caused varying degrees of environmental pollution, leading to the gradual deterioration of aquaculture diseases. In order to eliminate diseases, some farmers excessively use antibiotics, which can have a significant impact on the survival of aquatic animals and plants. The application of microorganisms can break away from the above situation and effectively optimize the ecological environment, which has a great promoting effect on the development of China's aquaculture industry.

Microbial composite agents for aquatic feed contain photosynthetic bacteria, nitrifying bacteria, lactic acid bacteria, lactobacillus, spore forming bacteria, white rot fungi, thermophilic streptococcus, etc. After fermentation, they are compounded with yeast, vitamins, amino acids and other nutrients. The aquatic feed fermented by microbial agents can effectively shorten the growth cycle of aquatic products, reduce the mortality rate of aquatic products, improve the survival rate, purify water quality, improve the aquaculture environment, effectively solve the problems of eutrophication and insufficient nutrients in water bodies, reduce the content of COD, BOD and toxic substances in water, prevent aquaculture diseases, inhibit the reproduction of harmful microorganisms and harmful algae, balance the micro ecological environment of aquaculture water bodies, and promote the healthy growth of aquaculture organisms. It is currently an ideal method for water treatment in aquaculture.

Application of composite microbial preparations to control and treat various water quality factors in aquaculture water bodies. The results showed that the optimal treatment effect was achieved at a concentration of 25 × 10 ^ -6~50 × 10 ^ -6. Microorganisms and fertilizer microorganisms in the aquaculture water were not only the main decomposers in the water, but also played a major role in material cycling. They were also important food sources for aquatic animals and fish. A large number of bacteria attach to the debris and are fed by fish, while more than half of the planktonic bacteria in the water usually aggregate into block or membrane like aggregates, which can be filtered and fed by fish. Fertilization and feeding in fish ponds can significantly increase the number, existing quantity, and production of bacteria. After using microbial agents in ponds, the water quality of aquaculture ponds can be improved, making the water more fertile, lively, tender, and refreshing; Promote the growth of beneficial algae, with fast, accurate, and vigorous fertilization and watering; Improve the production of aquatic animals such as fish, shrimp, and crabs.

4、 The Future Development Strategy of Deshen

With the rapid development of Internet technology, new energy, 5G technology and quantum science, green development has become the main tone of future development, and environmental harmony and human health are the main foundation of green development. Deshen Microbial Technology Co., Ltd. conforms to the development concept of the times and will definitely leverage its product advantages in green development. With the joint efforts of experts and professors in cooperation, we will overcome difficulties in the following five aspects and contribute to the green development strategy of the Party Central Committee and the State Council.

1. Demonstration of the Weight Loss, Quality Improvement and Efficiency Enhancement Effect of Deshen Microbial Agent in Major Vegetable Planting Bases
Deshen microbial agents have been comprehensively tested and promoted on major vegetable varieties, achieving remarkable demonstration effects, solving the drawbacks of long-term excessive use of fertilizers and pesticides such as soil degradation, excessive fertilizer, soil borne diseases, and low quality. Vegetables grown with Deshen microbial agents have restored the original flavor of the products, and various drug residues have been eradicated. The investment return rate of the product is as high as 5 times, which is an effective means to improve the planting conditions of large-scale protected areas in our province and enhance the quality competitiveness of agricultural products. It is suggested that the provincial party committee and government increase policy support in product promotion, so that more vegetable farmers can enjoy the fruits of technological progress and the general public can eat green vegetables with peace of mind.

2. Demonstration of Deshen microbial agent on super high yield of soybean and peanut

The strain of Deshen microbial inoculum contains natural growth promoting substances of leguminous plants, which can greatly improve the yield and quality of leguminous plants such as soybeans and peanuts. In 2020, the yield increase of field trials will reach 25-100%, and the maximum yield increase of peanuts will be more than 80%, reaching or exceeding the planting mode of plastic film mulch. Farmers' income will increase significantly, and the yield increase of soybeans will be nearly doubled. The economic benefits are very gratifying. Protein is an important guarantee for improving the quality of life of citizens, and leguminous plants such as peanuts and soybeans are the main sources of plant protein. Soybeans are also a scarce protein feed for aquaculture. So, the popularization of the company's products will greatly alleviate the problem of plant protein shortage in China.

3. Demonstration of the emission reduction effect of Deshen microbial agent on farmland in the Liaohe River Basin (Liaoning section)

The main principle of using Deshen microbial agents to increase the yield of field crops is to improve the fertilizer utilization efficiency of crops, which can increase the nitrogen fertilizer utilization efficiency by about 50% and the phosphorus fertilizer utilization efficiency by about 40%. At the same time, it also has the function of decomposing phosphorus, potassium and nitrogen fixation, greatly reducing the excessive nitrogen and phosphorus discharge from fertilizers into the water bodies of planting areas and significantly reducing the environmental damage caused by fertilizer loss in agricultural production. The Liao River is the mother river of the people of Liaoning, but it is the most polluted river in China's inland rivers, with severe loss of ecological functions. The use of Deshen microbial agents in farmland within a 10 kilometer radius of the Liao River basin can reduce nitrogen and phosphorus emissions from agriculture and alleviate the pressure of pollution control in the Liao River.

4. Construction of Technology and Demonstration Projects for Resource Utilization of Livestock Manure (Achieving Zero Emissions)

The breeding industry is the primary source of agricultural non-point source pollution. The loss and discharge of livestock manure are also important sources of pollution in the Liaohe River and groundwater. Along with fecal pollution, there is also a waste of undigested food resources in the feces. If manure is simply fertilized, the existing farmland area cannot bear it, and there are also many problems with biogas fermentation treatment. So it is necessary to open up new models for the resource utilization of manure to completely eliminate manure pollution. The conversion of grass rot fungus cultivation materials by mixing manure, biogas waste liquid and straw is a research project with great potential for development. While treating manure and straw, the raw materials required for grass rot fungus cultivation can be produced. Through the cultivation of grass rot fungi (such as Agaricus bisporus and Agaricus bisporus), a huge new industry can be created. Liaoning is a major province in edible mushroom production, mainly producing wood rot fungi (such as shiitake mushrooms, shiitake mushrooms, and black fungus). With the popularization of forest logging restrictions, cultivation materials will become increasingly scarce. Developing grass rot fungi is the only choice to achieve a win-win situation.

5. Application demonstration of Deshen microbial agent in antibiotic free aquaculture

Antibiotics not only prevent and treat diseases in aquaculture, but also promote animal growth, which has significant side effects on the quality of environmental products. Corresponding alternative products are necessary for its application control. At present, Deshen microbial agents, medicinal fungus Sanghuang, and traditional Chinese medicine Huangqi are all alternatives to antibiotics. Expanding application demonstrations and formulating breeding standards have urgent significance for improving the quality of livestock products. It is suggested that the government increase research investment in this field and promote the rapid development of antibiotic free aquaculture.
The company adheres to:

Our goal is to restore the original ecology of the environment, restore the original ecology of the soil, and create the original ecology of food.

Take from the people, use for the people, serve the people, and benefit the people!